Fluorescence In Situ Hybridization (FISH): Methods and Protocols (Methods in Molecular Biology Book 2784)

This volume explores the latest techniques and protocols used by researchers to address unique biological questions, model organisms not typically studied by Fluorescent In Situ Hybridization (FISH), protocols combining FISH with immunofluorescence (FISH-IF), and high-throughput experiments. The chapters in this book are divided into two parts: RNA FISH protocols and DNA FISH protocols. Part One covers methods for designing OligoPaint probes and studying distinct aspects of RNA biology such as transcription and splicing dynamics, and mRNA and small RNA expression and localization. Part Two discusses DNA repair dynamics, gene compaction, and chromatin conformation and gene rearrangements in plants, insects, and mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and thorough, Fluorescence In Situ Hybridization (FISH): Methods and Protocols is a valuable resource that will benefit the broader scientific community in their studies and understanding of this important field. 

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Enzymes: A Practical Introduction to Structure, Mechanism, and Data Analysis 3rd Edition

ENZYMES

A complete and approachable introduction to the study of enzymes, from theory to practice

Enzymes catalyze the bulk of important biological processes, both metabolic and biochemical. They are specialized proteins whose function is determined by their structure, understanding which is therefore a key focus of biological, pharmacological, and agrarian research, among many others. A thorough knowledge of enzyme structure, pathways, and mechanisms is a fundamental building block of the life sciences and all others connected to them.

Enzymes offers a detailed introduction to this critical subject. It analyzes enzyme proteins at the structural level and details the mechanisms by which they perform their catalyzing functions. The book’s in-depth engagement with primary literature and up-to-date research allows it to continuously deploy illustrative examples and connect readers with further research on key subjects. Fully updated after decades as the standard text, this book unlocks a thriving field of biological and biochemical research.

Readers of the third edition of Enzymes will also find:

• Expanded chapters on steady-state and transient-state enzyme kinetics, structural components of enzymes, and more
• New chapters on enzyme regulation, enzyme-macromolecule interactions, enzyme evolution, and enzymes in human health
• Key Learning Points at the beginning of each chapter to assist students and instructors

Enzymes promises to continue as the standard reference on this subject for practitioners of the life sciences and related fields in both academia and industry.

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Biochemistry For Dummies 3rd Edition

It’s alive! It’s alive! (Thanks to biochemistry, that is.) 

Biochemistry is the science of the chemical processes that allow for…well…life. If it moves, breathes, eats, or sleeps, biochemistry can probably explain how. So, it stands to reason that the fundamentals of biochemistry can get a little complicated. 

In Biochemistry For Dummies, you’ll explore the carbons, proteins, and cellular systems that make up the biochemical processes that create and sustain life of all kinds. Perfect for students majoring in biology, chemistry, pre-med, health-services, and other science-related fields, this book tracks a typical college-level biochemistry class. It simplifies and clarifies the subject with easy-to-follow diagrams and real-world examples. You’ll also get: 

• Explorations of cell biology, carbohydrates, proteins, lipids, and other fundamental building blocks of life 
• Discussions of the basic structures common to all living organisms 
• Treatments of the microscopic details of life that make us all tick 

If you’re looking for a hand with some of the trickier parts of biochemistry―or you just need an accessible overview of the subject―check out Biochemistry For Dummies today!  

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Principles Of Physical Biochemistry 2nd Edition

The Second Edition of Principles of Physical Biochemistry provides the most current look at the theory and techniques used in the study of the physical chemistry of biological and biochemical molecules—including discussion of mass spectrometry and single-molecule methods. As leading experts in biophysical chemistry, these well-known authors offer unique insights and coverage not available elsewhere. Physical techniques currently used by practicing biochemists, including new chapters dedicated to extended material on mass spectrometry and single-molecule methods are included. The book’s streamlined organization groups all hydrodynamic methods in Chapter 5 and combines Raman spectroscopy with the spectroscopy section. Relevant problems and applications help readers develop critical-thinking skills that they can apply to real biochemical and biological situations facing professionals in the industry.


Biological Macromolecules; Thermodynamics and Biochemistry; Molecular Thermodynamics; Statistical Thermodynamics; Methods for the Separation and Characterization of Macromolecules; X-Ray Diffraction; Scattering From Solutions of Macromolecules; Quantum Mechanics and Spectroscopy; Absorption Spectroscopy; Linear and Circular Dichroism; Emission Spectroscopy; Nuclear Magnetic Resonance Spectroscopy; Macromolecules in Solution: Thermodynamics and Equilibria; Chemical Equilibria Involving Macromolecules; Mass Spectrometry of Macromolecules; Single-Molecule Methods.

A useful reference for biochemistry professionals or for anyone interested in learning more about biochemistry.

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Harper’s Illustrated Biochemistry, Thirty-Second Edition 32nd Edition

Gain a thorough understanding of the principles of biochemistry as they relate to clinical medicine

A Doody’s Core Title for 2024 & 2023!

The Thirty-Second Edition of Harper’s Illustrated Biochemistry combines top-quality full-color illustrations with authoritative integrated coverage of biochemical disease and clinical information. Featuring numerous medically relevant examples, this respected text presents a clear, succinct review of the fundamentals that every student must understand in order to succeed in medical school.

All 58 chapters help you understand the medical relevance of biochemistry.

• Full-color presentation with 600+ illustrations
• Chapters have been updated to reflect the latest information
• Case studies emphasize the clinical relevance of biochemistry
• Review questions follow each of the 11 sections
• Boxed objectives define the goals of each chapter
• Tables encapsulate important information
• Each chapter contains a section on biomedical importance and a summary of the topics covered

Applauded by medical students for its current and engaging style, Harper’s Illustrated Biochemistry is an essential for USMLE review and the single best reference for learning the clinical relevance of any biochemistry topic.

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Biochemistry 2nd Edition

Biochemistry Second Edition, is a single-semester text designed for undergraduate non-biochemistry majors. Accessible, engaging, and informative, it is the perfect introduction to the subject for students who may approach chemistry with apprehension. Its unique emphasis on metabolism and its kinetic underpinnings gives the text up-to-the-minute relevance for students investigating current public health concerns, such as obesity and diabetes. Biochemistry Second Edition will encourage students to explore the basics of chemistry and its influence on biological problems.

Key Features:

• Provides an understanding of (mostly) enzymatic reactions that are responsible for the function and maintenance of living things.

• This innovative text for non-biochemistry majors includes introductory material at the beginning of each chapter that contextualizes chapter themes in real-life scenarios.

• Online supporting materials with further opportunities for research and investigation.

• Synthesis questions at the end of each chapter that encourage students to make connections between concepts and ideas, as well as develop critical-thinking skills.

About the Author:

Raymond S. Ochs is a biochemist with a career-long specialty in metabolism spanning 30 years. Previously, he has written the textbook Biochemistry, contributed the metabolism chapters to another text, Principles of Biochemistry, and co-edited a collection of articles published as Metabolic Regulation, and the recent monograph Metabolic Strucure and Regulation. His research interests concern major pathways of liver and muscle, including glycolysis, gluconeogenesis, ureogenesis, fatty acid metabolism, glycogen metabolism, and control by cAMP, Ca2+, diacylglycerol, and AMPK. He is currently professor of pharmacy at St. John’s University in New York, teaching biochemistry, physiology, and medicinal chemistry.

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Medical Biochemistry 5th Edition

Now fully revised, this acclaimed textbook efficiently links basic biochemistry with the day-to-day practice of medicine. You will learn basic science concepts and see them illustrated by clinical cases that describe patients you will likely encounter in your clinical training. You will also learn about the use of laboratory tests to diagnose and monitor the most important conditions. Brought to you in a thorough yet accessible manner, this new edition of Medical Biochemistry highlights the latest developments in regulatory and molecular biology, signal transduction, biochemistry and biomarkers of chronic disease, and bioinformatics and the ‘-omics’. It highlights the most important global medical issues: diabetes mellitus, obesity and malnutrition, cancer and atherosclerotic cardiovascular disease, and addresses the role of nutrition and exercise in medicine.

• Featuring a team of expert contributors that includes investigators involved in cutting-edge research as well as experienced clinicians, this book offers a unique combination of research and clinical practice tailored to today’s integrated courses.
  • Read organ-focused chapters addressing the biochemistry of the bone, kidney, liver, lungs and muscle; and system-focused ones addressing the biochemistry of the immune and endocrine systems, neurochemistry and neurotransmission, and cancer

   

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Clinical Biochemistry and Metabolic Medicine 8th Edition

Whether you are following a problem-based, an integrated, or a more traditional medical course, clinical biochemistry is often viewed as one of the more challenging subjects to grasp. What you need is a single resource that not only explains the biochemical underpinnings of metabolic medicine, but also integrates laboratory findings with clinical p

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Biochemistry (Lippincott’s Illustrated Review) 5th Edition

Thoroughly updated for its Fifth Edition, Lippincott’s Illustrated Reviews: Biochemistry enables students to quickly review and assimilate large amounts of complex information through powerful visual resources essential to mastery of difficult biochemical concepts. Its signature outline format, full-color illustrations, end-of-chapter summaries, and USMLE-style review questions make it one of the most user-friendly books in the field. New features include expanded coverage of molecular biology.

A companion website offers fully searchable online text and additional USMLE-style questions for students and an image bank for faculty.

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Harper’s Illustrated Biochemistry (LANGE Basic Science) 26th Edition

Extensively revised and updated, this authoritative biochemistry text is known worldwide for its comprehensive and up-to-date coverage. Extensively illustrated and user-friendly, the text offers examples pf how knowledge of biochemistry is essential for understanding the molecular basis of health and disease. The 26th edition also features expanded content on results of the Human Genome Project. Perfect as both text and USMLE review.

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